Improvement of the production of poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate‐co‐4‐hydroxybutyrate) terpolyester by manipulating the culture condition

BACKGROUND: The aim of this work is to enhance the production of terpolyester poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate‐co‐4‐hydroxybutyrate) (P(3HB‐co‐3HV‐co‐4HB)) produced by a locally isolated bacterium, Cupriavidus sp. USMAA2‐4. The monomer composition was varied by supplementing different carbon precursors and by manipulating the culture condition through one‐stage cultivation. The effect of C/N ratio and different concentrations of carbon source and precursors were investigated in order to produce higher content of this terpolyester. Although research on this biodegradable polyester is abundant, studies on terpolyester P(3HB‐co‐3HV‐co‐4HB) are still limited. RESULTS: Supplementation of oleic acid in accumulation medium increased the bacterial growth and polyhydroxyalkanoate (PHA) accumulation. It was also shown that medium consisting of assorted carbon precursors at C/N 20 gave relatively high dry cell weight and P(3HB‐co‐3HV‐co‐4HB) content. Various compositions of terpolyester were obtained when the concentration of oleic acid and 4HB precursors were manipulated. The combination of oleic acid with γ‐butyrolactone and 1‐pentanol was found to be the best combination to produce high PHA content (81 wt%). The composition of monomer in P(3HB‐co‐3HV‐co‐4HB) was produced in the range 8–13 mol% for 3HV and 9–24 mol% for 4HB, respectively. CONCLUSIONS: The production of P(3HB‐co‐3HV‐co‐4HB) in shake‐flasks successfully produced 81 wt% of PHA content. This manipulated culture condition can be used at larger scale to provide modeling for the production of terpolyester in a bioreactor. Copyright © 2012 Society of Chemical Industry     

Essais saoudiens sur les ciments importés

Au fur et à mesure que le développement s'accélère en Arabie saoudite, les matériaux importés deviennent de plus en plus nombreux et entrent en concurrence les uns avec les autres. Le Materials Testing Laboratory de Riyad a procédé, il ny a pas très longtemps, à une étude comparative d'échantillons de ciments importés et de ciments fabriqués dans le pays. S'ils concernent avant tout les chimistes du ciment, les résultats n'en présentent pas moins un intérêt plus général.     

Characterization of vanadyl phthalocyanine based surface-type capacitive humidity sensors

This study presents the fabrication and characterization of novel surface-type capacitive humidity sensors using vanadyl phthalocyanine (VOPc) as the active material. The devices, which comprise three different thicknesses,have been fabricated using the thermal evaporation technique. A thin film of VOPc is deposited on thoroughly cleaned glass substrates with pre-pattemed Ag electrodes. The capacitive effect of the samples under humidity has been in-vestigated. Comparison of the samples with different thicknesses shows that the thinnest device seems more sensitive towards humidity. The humidity dependent capacitance properties of the sensor make it beneficial for use in commercial hygrometers.     

High quality biodiesel and its diesel engine application: A review

The continuous increasing demand for energy and the diminishing tendency of petroleum resources has led to the search for alternative renewable and sustainable fuel. Biodiesel is best substitute for petro-diesel and also most advantageous over petro-diesel for its environmental friendliness. The quality of biodiesel fuel was found to be significant for its successful use on compression ignition engines and subsequent replacement of non-renewable fossil fuels. Conventional biodiesel separation and purification technologies were noticed to yield lower quality biodiesel fuel with resultant excessive energy and water consumptions. Membrane technology showed more potential for effective and efficient separation and purification of biodiesel. This technology need be explored for the attainment of better quality biodiesel fuels. This paper reviews the technologies used for the biodiesel separation and purification, biodiesel quality, and its effects on diesel engines. Biodiesel biodegradability, lubricity, stability, economic importance, and gaseous emissions have been discussed.     

Full-Scale Test of Continuity Diaphragms in Skewed Concrete Bridge Girders

Continuity diaphragms used in prestressed girder bridges on skewed bents have caused difficulties in detailing and construction. The results of the field verification for the effectiveness of continuity diaphragms for skewed, continuous, and prestressed concrete girder bridges are presented. The current design concept and bridge parameters that were considered include skew angle and the ratio of beam spacing to span (aspect ratio). A prestressed concrete bridge with continuity diaphragms and a skewed angle of 48° was selected for full-scale test by a team of engineers from Louisiana Department of Transportation and Development and the Federal Highway Administration. The live load tests performed with a comprehensive instrumentation plan provided a fundamental understanding of the load transfer mechanism through these diaphragms. The findings indicated that the effects of the continuity diaphragms were negligible and they can be eliminated. The superstructure of the bridge could be designed with link slab. Thus, the bridge deck would provide the continuity over the support, improve the riding quality, enhance the structural redundancy, and reduce the expansion joint installation and maintenance costs.     

Quadrature rules for numerical integration based on Haar wavelets and hybrid functions

In this paper Haar wavelets and hybrid functions have been applied for numerical solution of double and triple integrals with variable limits of integration. This approach is the generalization and improvement of the methods (Siraj-ul-Islam et al. (2010) [9]) where the numerical methods are only applicable to the integrals with constant limits. Apart from generalization of the methods [9], the new approach has two major advantages over the classical methods based on quadrature rule: (i) No need of finding optimum weights as the wavelet and hybrid coefficients serve the purpose of optimal weights automatically (ii) Mesh points of the wavelets algorithm are used as nodal values instead of considering the n nodes as unknown roots of polynomial of degree n. The new methods are more efficient. The novel methods are compared with existing methods and applied to a number of benchmark problems. Accuracy of the methods are measured in terms of absolute errors.     

Chemical composition,antioxidative capacity and interactive antimicrobial potency of Satureja khuzestanica Jamzad essential oil and antimicrobial agents against selected food‐related microorganisms

This study aimed to determine the chemical composition, antimicrobial and antioxidative activity of Satureja khuzestanica Jamzad essential oil. The oil was analysed by GC and GC/MS. Twenty‐eight constituents were identified. The oxygenated monoterpenes (78.22%) were the principal compound group. Among them, carvacrol (53.86%) and thymol (19.84%) were the most abundant constituents. The oil exhibited an acceptable antimicrobial activity against most of the tested microorganisms. The checkerboard method was applied to determine fractional inhibitory concentration indices (FICIs) to interpret the synergetic, additive, indifference or antagonistic interactions between essential oil and each of antimicrobials (lysozyme, ciprofloxacin, fluconazole and amphotericin B) against food‐related microorganisms. The synergetic phenomenon (FICI ≤ 0.5) was observed in majority of combinations with the exception of the essential oil and lysozyme. The oil exhibited good 1,1‐diphenyl‐2‐picrylhydrazyl radical scavenging activity (IC₅₀ = 28.71 μg mL^?1). Also, the oil had strong antioxidative activity in β‐carotene‐linoleic acid assay relative antioxidant activity (RAA% = 95.45). This study demonstrated that the essential oil has beneficial biological properties and its simultaneous application with standard antimicrobials against food‐related microorganisms result in reduction in inhibitory doses of the antimicrobials in vitro.     

Interval timing accuracy and scalar timing in C57BL/6 mice.

In many species, interval timing behavior is accurate—appropriate estimated durations—and scalar—errors vary linearly with estimated durations. Whereas accuracy has been previously examined, scalar timing has not been clearly demonstrated in house mice (Mus musculus), raising concerns about mouse models of human disease. The authors estimated timing accuracy and precision in C57BL/6 mice, the most used background strain for genetic models of human disease, in a peak-interval procedure with multiple intervals. Both when timing 2 intervals (Experiment 1) or 3 intervals (Experiment 2), C57BL/6 mice demonstrated varying degrees of timing accuracy. An important finding was that, both at the individual and group levels, their precision varied linearly with the subjective estimated duration. Further evidence for scalar timing was obtained using an intraclass correlation statistic. This is the first report of consistent, reliable scalar timing in a sizable sample of house mice, thus validating the peak-interval procedure as a valuable technique, the intraclass correlation statistic as a powerful test of the scalar property, and the C57BL/6 strain as a suitable background for behavioral investigations of genetically engineered mice modeling disorders of interval timing. (PsycINFO Database Record (c) 2010 APA, all rights reserved)